CircPlant: An Integrated Tool for circRNA Detection and Functional Prediction in Plants / 基因组蛋白质组与生物信息学报·英文版

The recent discovery of circular RNAs (circRNAs) and characterization of their functional roles have opened a new avenue for understanding the biology of genomes. circRNAs have been implicated to play important roles in a variety of biological processes, but their precise functions remain largely elusive. Currently, a few approaches are available for novel circRNA prediction, but almost all these methods are intended for animal genomes. Considering that the major differences between the organization of plant and mammal genomes cannot be neglected, a plant-specific method is needed to enhance the validity of plant circRNA identification. In this study, we present CircPlant, an integrated tool for the exploration of plant circRNAs, potentially acting as competing endogenous RNAs (ceRNAs), and their potential functions. With the incorporation of several unique plant-specific criteria, CircPlant can accurately detect plant circRNAs from high-throughput RNA-seq data. Based on comparison tests on simulated and real RNA-seq datasets from Arabidopsis thaliana and Oryza sativa, we show that CircPlant outperforms all evaluated competing tools in both accuracy and efficiency. CircPlant is freely available at http://bis.zju.edu.cn/circplant.

Effects of early intelligent feedback rehabilitation training system of lower extremities on walking ability in stroke patients with hemiplegia / 中国脑血管病杂志

Objective To observe the effects of early intelligent feedback rehabilitation training system of lower extremities on walking ability in stroke patients with hemiplegia. Methods Forty post-stroke hemiplegic patients (onset 0. 05). Four weeks after treatment, the FMA-L scores were improved in both the intelligent and conventional rehabilitation groups compared to before treatment (P<0. 01);the differences were 20. 0 ± 6. 2 and 5. 1 ± 1. 6 respectively before and after treatment. The degree of improvement of the intelligent rehabilitation group was better than that of the conventional rehabilitation group (P<0. 01). Holden walking function classification showed that only the intelligent rehabilitation group was improved as compared to before treatment (P<0. 01). Four weeks after treatment ,the strength and tone of the tibialis anterior muscle and gastrocnemius muscle of both groups were increased compared to before treatment (P <0. 01 ). The differences of the strength and tone of the tibialis anterior muscle were 20 +7 and 12 +4μV respectively in the rehabilitation groups before and after treatment,and those were 12+4 and 9+3μV in the conventional rehabilitation group;the differences of the strength and tone of the gastrocnemius muscle of the intelligent rehabilitation group were 25+8 and 19+6μV respectively before and after treatment,and those of the conventional rehabilitation group were 10+3 and 11+2μV respectively. There was significant difference between the intelligent rehabilitation group and the conventional rehabilitation group (P <0. 01 ). Conclusion The treatment of early intelligent feedback rehabilitation training system of the lower extremities may significantly improve the walking ability of the lower extremities in stroke patients with hemiplegia.

Effects of Motor Imaginary Therapy Combined with Occupational Therapy on Function of Upper Limbs and Hands for Stroke Patients with Hemiplegia / 中国康复理论与实践

@#Objective To explore the effects of motor imaginary therapy combined with occupational therapy on the function of upperlimbs hands and activities of daily living for stroke patients with hemiplegia. Methods 62 stroke patients with hemiplegia were divided intoobservation group (n=31) and control group (n=31). Both groups were treated with basic medication and occupational therapy while the observationgroup was treated additionally with motor imaginary therapy. The function of upper limbs and hands was assessed by Fugl-MeyerAssessment (FMA), and activities of daily living was assessed by Functional Independence Measurement (FIM) before and 2 months aftertreatment. Results There were significant difference between the two groups in the scores of FMA in the function of upper limbs and hands,and FIM 2 months after the treatment (P<0.01). Conclusion Motor imaginary therapy combined with occupational therapy can effectivelyimprove the functional outcomes of upper limbs and hands for stroke patients with hemiplegic, and can even effectively improve the functionalactivities of daily living.